MICROPROPAGATION OF OF ORCHIS CATASETUM - A RARE AND ENDANGERED ORCHID

Published in Scientific Papers. Series B, Horticulture, Volume LVIII
Written by Mohammad Vali FAKOURI GHAZIANI, Ahmad BAKER, Naser NEGAHDAR, Behzad KAVIANI

The application of modern biotechnology for mass propagation of endangered species needs to develop a proper in vitro protocol. Here, a protocol was developed for high frequency in vitro multiplication of an endangered orchid, Orchis catasetum. Protocorms, as explants were cultured on Murashige and Skoog (MS) medium fortified with different concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) either individually or in combination. A combination of 0.5 mg l1 BA and 0.5 mg l-1 NAA was found to be suitable for maximum protocorm-like bodies (PLBs) regeneration (20.40/plantlet). The maximum number of root (7.16/plantlet) and leaf (10.10/plantlet), also the highest plant height (114.20 mm/plantlet) and root length (193.40 mm/plantlet) were obtained on MS medium supplemented with 0.5 mg l1 BA a long with 0.5 mg l-1 NAA. Plantlets with well-developed leaves and roots were transplanted to pots filled with perlite, wood pieces, ionolite and mineral cartridge shell (1:1:1:1), also perlite individually and transferred to the greenhouse. Upon ex vitro transfer, 100% of plants survived.

[Read full article] [Citation]

Fakouri Ghaziani M. V., Baker A., Negahdar N., Kaviani B. 2014, MICROPROPAGATION OF OF ORCHIS CATASETUM - A RARE AND ENDANGERED ORCHID. Scientific Papers. Series B, Horticulture, Volume LVIII, Print ISSN 2285-5653, 317-322.


The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.
© 2012 SCIENTIFIC PAPERS. Series B. Horticulture. All Rights Reserved. To be cited: SCIENTIFIC PAPERS. Series B. Horticulture
Powered by INTEL TECH DEVELOPMENT.