Published in Scientific Papers. Series B. Horticulture, Vol. LVI
Written by Adrian PETICILĂ, Florin STĂNICĂ, Roxana MADJAR, Oana VENATDUMITRIU
An effective in vitro culture system for mature stem segments of Actinidia arguta was established. The 1.2 cm nodal segments, cut from young shoots, was sterilized and established in vitro. The successfully induced culture was achieved on three types of growing media standing for three experimental variants were prepared according to the reference bibliography. The first culture medium variant is the classic MS with unchanged components, the second is the modified MS medium with a double quantity of ammonium nitrate (2N) and the third variant is the modified MS medium with a triple quantity of ammonium nitrate (3N) in the composition of macroelement salt solutions. These changes result from the study of reference literature where the ammonium nitrate was modified in order to boost the vegetative growth of explants. This experimental scheme was applied for the male and female genitor of Actinidia arguta. The shoots of micro cuttings were rooted in ½ MS supplemented with 0.5mg/l IBA, 12.5g/l sucrose. Regenerated shoots successfully acclimatized to vegetation house.
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{tag}meta name="citation_title" content=" Micropropagation of baby kiwi (Actinidia arguta) using mature stem segments"{/tag} {tag}meta name="citation_journal_title" content="Scientific Papers. Series B. Horticulture"{/tag} {tag}meta name="citation_publication_date" content="2012"{/tag} {tag}meta name="citation_author" content="Adrian PETICILĂ" {/tag} {tag}meta name="citation_author" content=" Florin STĂNICĂ" {/tag} {tag}meta name="citation_author" content=" Roxana MADJAR" {/tag} {tag}meta name="citation_author" content=" Oana VENATDUMITRIU " {/tag}
Actinidia arguta
