RESEARCH ON THE PREPARATION A PROTOCOL OF THE DIRECT ORGANOGENESIS THROUGH "IN VITRO" CULTURE TECHNIQUES TO HELIANTHUS TUBEROSUS L. (JERUSALEM ARTICHOKE)

Published in Scientific Papers. Series B, Horticulture, Volume LX
Written by Silvana DĂNĂILA-GUIDEA, Ricuţa-Vasilica DOBRINOIU, Luminiţa VIȘAN

In the context of the current social and economic effects of the global crisis, it manifests itself with greater intensity and in one of the most important sectors of the Romanian economy - agriculture. Modern technique has transformed farming from a craft mastered in practice daily in a complex science that allows routing of the factors of vegetation and the transition to industrial agriculture. In this paper there will be presented arguments that underline the use of Jerusalem Artichoke (Sun root). Based on general principles of the techniques of "in vitro" culture, whose applicability was verified in the case of most species, our research aims to establish the optimal conditions for developing crops with pronounced morphogenetic capacity. The planting material at tuberous roots of artichoke was studied with greater efficiency by biotechnological methods. The working protocol was developed as a result of research undertaken on apexes "in vitro" cultivation taken from sprouts obtained by forcing the tubers in cold weather to grow shoots in the presence of additional fitohormones (BAP, K, NAA). These tubers were used to fulfill micropropagation requirements through an efficient growth "in vitro" that can succesfully be adapted to improved micropropagation for the varieties of Jerusalem artichoke.

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DĂNĂILA-GUIDEA S., DOBRINOIU R.V., VIȘAN L. 2016, RESEARCH ON THE PREPARATION A PROTOCOL OF THE DIRECT ORGANOGENESIS THROUGH "IN VITRO" CULTURE TECHNIQUES TO HELIANTHUS TUBEROSUS L. (JERUSALEM ARTICHOKE). Scientific Papers. Series B, Horticulture, Volume LX, Print ISSN 2285-5653, 175-184.


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