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Published in Scientific Papers. Series B, Horticulture, Volume LXI
Written by George Nicolae CAZAN, Florin TOMA

These researches highlights the advances made on various aspects of herbaceous peony (Paeonia lactiflora Pall.) tissue culture, including the in vitro culture of underground buds, leaves and petioles, the induction of cluster buds, and callus induction. Tissue culture has the ability of speeding up the rate of propagation, reducing breeding time and meeting the needs of mass production. Problems that are currently being faced in herbaceous peony tissue culture are highlighted and possible viable solutions are provided. More research is needed to make mass production of peony more commercially successful. Optimization of procedures are necessary, from selection of explants, decontamination, screening of medium, application of plant growth regulators (PGRs), induction of callus and shoots, subculture, rooting, and to final transplanting. The major goal of these studies is to evaluate shoot induction ability of peony explants and PGRs. The development of micropropagation methods for peonies is necessary to not only overcome this problem but also accelerate peony breeding progress. Tissue culture is one of the most effective approaches for rapid propagation of plants and is also providing a new approach for plant breeding in crops, ornamentals, fruits and vegetables. Effect of Gelling Agents on Tissue Culture of Herbaceous Peony. Comparison of Basal Medium in Tissue Culture of Herbaceous Peony. Effect of Pretreatment of Underground Buds on Tissue Culture of Herbaceous Peony. Investigation of Callus and Shoot Induction Ability of Flower Tissue. Effect of PPM on Decontamination and Rescue of Explants. Root Induction and Transplanting of Herbaceous Peony In Vitro.

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