MOLECULAR DETECTION OF BLACK ASPERGILLUS AND PENICILLIUM SPECIES FROM DEALU MARE VINEYARD

Published in Scientific Papers. Series B, Horticulture, Volume LXII
Written by Camelia Filofteia DIGUȚĂ, Radu Cristian TOMA, Călina Petruța CORNEA, Florentina MATEI

The aim of this study was to determine the incidence and diversity of black Aspergillus and Penicillium species on white and red grape varieties in Dealu Mare vineyard (in two vilticultural centres - Valea Calugareasca and Pietroasa), during the harvest time of 2014 and 2015. A total of 61 fungal strains belonging to black Aspergillus (28 strains) and Penicillium (33 strains) were isolated. An RFLP analysis of the 5.8 S-ITS region was performed by using different combinations of endonucleases for molecular identification of fungal isolates at species level. A. niger (accounting 60.71% of all isolates) and P. expansum (66.66%) were the predominant species identified, followed by A. tubingensis (17.85%) and P. chrysogenum (21.21%). Higher biodiversity of fungal isolates has been found in Pietroasa Centre than from Valea Calugareasca.

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DIGUTA C.F., TOMA R.C., CORNEA C.P., MATEI F. 2018, MOLECULAR DETECTION OF BLACK ASPERGILLUS AND PENICILLIUM SPECIES FROM DEALU MARE VINEYARD. Scientific Papers. Series B, Horticulture, Volume LXII, Print ISSN 2285-5653, 305-310.


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