PRELIMINARY TESTING OF SRAP PRIMERS IN FOUR RANUNCULACEAE SPECIES FROM ROMANIA

Published in Scientific Papers. Series B, Horticulture, Volume LXII
Written by Cristina D. KELEMEN, Orsolya BORSAI, Katalin SZABO, Doina CLAPA, Monica HÂRŢA, Doru PAMFIL

The main aim of this paper was to identify the most reliable primer pairs out of the 64 used in order to investigate the genetic diversity of four medicinal Ranunculaceae species as follow: Aconitum moldavicum, Anemone transsilvanica, Ranunculus carpaticus and R. platanifolius. Amplified products were separated on 1.6% agarose gel and a total number of 886 DNA fragments were visualized by EtBr staining. After primer combinations screening, 27 primer pairs were selected based on clarity, reproducibility of the amplified bands and high rates of polymorphism for further research. The selected SRAP primer pairs resulted in 553 informative fragments with an average number of 20.5 per primer combination. The most efficient primer combinations were Me1/Em1, Me3/Em3, Me7/Em4, Me7/Em5, Me7/Em8, Me8/Em1 and Me8/Em5 which generated a total number of 201 visible DNA fragments. The current research represents a preliminary study for further conservation perspectives regarding the genetic diversity of several medicinal plants belonging to the Ranunculaceae family.

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KELEMEN C.D., BORSAI O., SZABO K., CLAPA D., HARTA M., PAMFIL D. 2018, PRELIMINARY TESTING OF SRAP PRIMERS IN FOUR RANUNCULACEAE SPECIES FROM ROMANIA. Scientific Papers. Series B, Horticulture, Volume LXII, Print ISSN 2285-5653, 629-636.


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