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Published in Scientific Papers. Series B, Horticulture, Volume LXII
Written by Mohammed Naithel RADHI, Cristina PETRISOR, Florica CONSTANTINESCU, Ioan ROSCA, Hattf Bazool FARHOOD, Hiba ABD- AL ADHEEM, Gheorghe STOIAN

The fungi from Trichoderma genus are a very large group of microorganisms that are present in nearly all agricultural soils and play a significant role in plant protection. Enzymes produced by species of genus Trichoderma constitute an important group of biotechnologically enzymes because of the versatility of its properties and ease of mass production. This study was focused on the effect different factors of antagonistic T. harzianum (ICCF 417) and T. koningii (ICCF 418) against pathogenic fungus F. oxysporum (ZUM 2407) by microbiologic and biochemical tests. There was different patterns in cell wall degrading enzymes production by Trichoderma isolates so production of chitinase was 113.93 % by T. harzianum comparative to T. koningii, the lipase was 38.53% in T.harzianum comparative to T. koningii while the protease was 91.43% in T. harzianum comparative to T. koningii. Both Trichoderma strains studied showed maximum lipase activity at pH 9, which was significantly higher than those at other pH levels. On the other hand, optimum pH for producing highest activity of protease was pH 6. A high level of chitinase activity was observed in the culture medium with pH 6. Our results showed that hydrolase activities studied in this experiment play an important role in pathogenic fungus F. oxysporum inhibition and the degree of effect is different.

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