Published in Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 1
Written by Doina CLAPA, Monica Hârța, Claudiu Ioan BUNEA, Lehel LUKÁCS, Eugenia HĂRȘAN, Gabriela ROMAN, Peter Alpar SOMSAI
This study was carried out to investigate the effect of three cytokines [6-benzyladenine (BA), meta-Topoline (mT), and zeatin (Z)] on the in vitro propagation of C. avellana. The culture medium used in all in vitro culture stages was McCown's Woody Plant Medium (WPM) supplemented with 100 mg/l Sequestrene 138.3% (w/v) sugar, solidified with 5 g/l. Plant agar and pH 5.8. At the initiation stage, single-node mini cuttings were inoculated on WPM medium supplemented with 2 mg/l Z, and the initiation percentage was 66.66%. The highest proliferation rate (2.21 ± 0.11) was observed on the culture medium supplemented with 4 mg/l BA followed by the culture medium supplemented with 4 mg/l Z (1.93 ± 0.03). The shortest shoots were measured on WPM medium with 4 mg/l BA, with an average length of 1.81 ± 0.08 cm, and the longest shoots (3.46 ± 0.02 cm) were obtained on WPM medium with 4 mg/l Z. The in vitro rooting percentage was 81.66 ± 0.39% when using the WPM medium with 1 mg/l Z. The rooted plants obtained in vitro showed an acclimation rate of 97.66%, and out of these, 96% survived under greenhouse conditions.
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{simplepopup popup="false" name="Citation9"}CLAPA D., M.H., BUNEA C.I., LUKACS L., HARSAN E., ROMAN G., SOMSAI P.A. 2023, EFFECTS OF CYTOKININS IN CORYLUS AVELLANA L. MICROPROPAGATION. Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 1, Print ISSN 2285-5653, 78-84. {/simplepopup}