Published in Scientific Papers. Series B. Horticulture, Vol. LVI
Written by Mădălin CHIUOARU, Gheorghita HOZA, Dan MANAFU
Cucumber mosaic virus (CMV) was first described in detail in 1916 on cucumber (simultaneously by Doolittle and Jagger) and other cucurbits, but is now known to occur worldwide in both temperate and tropical climates, affecting many agricultural and horticultural crops. Development of genetic resistance to CMV in many vegetables has made a valuable contribution for disease management of this important virus disease. There are some CMV resistant (tolerant) cucumber varieties available that produce a good crop, but most other cucurbits are susceptible to CMV. The screening of the 37 field samples collected from six species of Cucurbitaceae were tested by TAS-ELISA, 13 samples were found to be infected by CMV. Among them. In IC-RT-PCR using the MAbs and specific primers in the region of the coat protein (CP) gene, samples shown that results obtained by TAS-ELISA gave one specific band about 500 nucleotides in length. The validity and reliability of the results of TAS-ELISA and IC-RT-PCR was confirmed by sequencing and phylogenetic analysis of nearly full-length CP genes of the isolates.
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{tag}meta name="citation_title" content=" Preliminary research concerning the identification of reistance genotypes on Cucurbitaceae family in artificial infection conditions with cmv (Cucumber mosaic virus)"{/tag} {tag}meta name="citation_journal_title" content="Scientific Papers. Series B. Horticulture"{/tag} {tag}meta name="citation_publication_date" content="2012"{/tag} {tag}meta name="citation_author" content="Mădălin CHIUOARU" {/tag} {tag}meta name="citation_author" content=" Gheorghita HOZA" {/tag} {tag}meta name="citation_author" content=" Dan MANAFU " {/tag}
Cucumber mosaic virus
