Published in Scientific Papers. Series B. Horticulture., Vol. LVII
Written by STUDY ON THE FREE AMINO ACID PROFILE OF LEAVES FOR GRAPES VARIETIES OF GALBENĂ DE ODOBEŞTI SORTOGROUP
Researches on amino acid profile carried out so far in the genus Vitis showed its great variability, suggesting importance of the genetic involvement in this variability This paper presents preliminary results of investigating the amino acid profile from leaves to the grape varieties of sortogroup Galbenă de Odobeşti. The grape varieties taken in the study were: Galbenă de Odobeşti, Bătută neagră, Zghihară de Huşi, Negru moale and Negru vârtos. For extraction of free amino acids from the leaves of the vine has been used the method Bieleski & Turner (1966) adjusted for grapevine, and separation technique was performed by thin-layer chromatography (TLC). Identification and quantitative estimation of free amino acids was performed using analysis and image processing software - ImageJ ver.1.46. The amount of free amino acids ranged between 18.76 mg / mg fresh leaf at Galbenă de Odobeşti variety and 14.33 mg / mg for Negru moale variety. In the varieties Galbenă de Odobeşti, Bătută neagră and Zghihară de Huşi the most abundant free amino acids were: aspartic acid (Asp), glutamic acid (Glu), serine (Ser), threonine (Thr), proline (Pro) and glycine (Gly). The predominant amino acids for Negru moale variety were: aspartic acid (Asp), serine (Ser), glycine (Gly), glutamic acid (Glu), threonine (Thr), proline (Pro) and arginine (Arg). Amino acids predominantly to Negru vârtos variety are: aspartic acid (Asp), glutamic acid (Glu), serine (Ser), threonine (Thr), proline (Pro) and arginine (Arg). Aspartic acid (Asp) is found in large quantities compared with other amino acids, representing 31.7% of total amino acids identified for Negru vârtos variety, 31.5% at Zghihară de Huşi variety, 30.5% fpr Negru moale variety, 27. 5% for Galbenă de Odobeşti variety and 25.3% for the variety Bătută neagră. Statistical ratio Pro / Arg and amino predominant ratio (Asp / Ser), genetically differentiate the Bătută neagră variety the other varieties belonging to the sortogroup Galbenă de Odobeşti, with a confidence interval of 95%. These preliminary data provide a basis for further research that can demonstrate that amino acid profile of the leaves can be used as method in discriminant analysis of grape varieties.
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amino acid profile
