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DETERMINATION ON VITAMIN C IN SEVERAL OLD ROMANIAN APPLE CULTIVARS BY HPLC DURING COLD STORAGE

Published in Scientific Papers. Series B. Horticulture., Vol. LVII
Written by Dan Petrişor MANAFU, Dorel HOZA, Ligia ION

High pressure liquid chromatographic (HPLC) methods were used for measurement of vitamin C and organic acid changes of forty old Romanian apple cultivars (“Prescurate”,“Gurguiate”,“Zori”,“Carla”,“Mohorat”, “Trotuse”, “Mere Tari”,”Mar Orbai” etc.) during cold storage. Harvested apples at the last stage of commercial ripeness were placed in perforated stored at 0°C temperature and 90-95% relative humidity for 60 days. Vitamin C content decreased in all cultivars but no significant differences were found in the most important of them from the beginning to the end of the storage. The highest share of total acids was contributed by citric acid. The high level of vitamin C was measured in the cultivars” Trotuse”, “Ancuta”,”Wachsman Sammeling”and “Wachsman Amalie”. Malic acid content of cultivars also decreased with storage time. Tartaric, oxalic and fumaric acid contents fluctuated during storage, but at the end of cold storage these organic acids had decreased in comparison to initial values.

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