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PRELIMINARY STUDY RELATED HIGHLIGHTING THE INHIBITORY EFFECT OF IN VITRO FUNGUS GROWTH MYCOSPHAERELLA GROSSULARIAE (AUERS.) LIND. BY SAPROPHYTIC FUNGI

Published in Scientific Papers. Series B. Horticulture., Vol. LVII
Written by Eugenia PETRESCU, Tatiana-Eugenia ŞESAN, Florica CONSTANTINESCU, Maria OPREA, Traian MANOLE, Irina IONESCU-MĂLĂNCUŞ

Mycosphaerella grossulariae (Auers.) Lind., one of the most important pathogenic fungi which affect the black currant ecological crops cultivated for alimentary supplements and phytotherapeutics drugs production. The present study brings the new data related to the possibility of „in vitro” vegetative growth inhibition of fungus mycelium using saprophytic fungi species. The species of fungi used for experimental trials was Trichoderma viride, Trichothecium roseum Link, Epicoccum nigrum Link. and Gliocladium roseum Bainier. The fungus was growth on several culture media for comparative testing and establishment of the most efficient medium for vegetative growth of the fungi. In the study we also follow the comparative approach doing by biometric measurements of the colonies in which work variant the pathogenic fungi had the high rate of growing. After the testing was made the PDA medium was selected for experimentation of inhibitory effect of the saprophytes. The method used for trials was the double cultures technique on the medium of agar which consists in inoculation of one media fragment of mycelium carrier separated from the agar medium of saprophytic fungi on some distance from pathogenic fungi. The study carried out had allowed the highlight of the fact that in all experimental variant used the saprophytic fungi had inhibits the Mycosphaerella grossulariae pathogen growing. The detailed analysis of the results we could reveal that the most inhibitory effect was done by T. viride followed by T. roseum and E.nigrum.

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