Published in Scientific Papers. Series B, Horticulture, Volume LIX
Written by Leonard BOROŞ, Tatiana Eugenia ŞESAN, Mariana Carmen CHIFIRIUC, Ionela DOBRIN, Beatrice IACOMI, Claudia COSTACHE
Although the vegetable fields is reduced in Romania, the production losses remain high and this concern is not owedonly to fungui, viruses, bacteria or insects, but also to nematodes, these last organisms being less known andacknowledged. Meloidogyne incognita (Kofoid & White, 1919) and Meloidogyne hapla (Chitwood, 1949) areconsidered important parasitic nematodes, but quite little studied in Romania, for different species of dicotyledonvegetables and this article demonstrates and compares the development and reproduction of these two species relatedto one of the most important vegetable cultures from the economic point of view. It seems that the Meloidogyne haplaspecies especially prefer the species from the Apiaceae family, unlike those belonging to Meloidogyne incognita whichdevelop a more intense shock on Brassicaceae and Solonaceae botanic family species. However, the eggs massesdetected for both root-knot nematodes species show a unitary type of distribution at the surface of the of roots corticalarea of all analyzed vegetable species. A contamination with the Meloidogyne incognita species, unusually high, hasbeen noticed for the first time in our country in Brassica oleracea species.The diagnosis through biomorphometry onsemi-permanent microscopic preparations, completed with the molecular biology techniques (restriction fragmentlength polymorphism - RFLPs ) led to the conclusion that the twocategories of diagnosis methods can be considered asbeing complementary.
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