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Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 1
Written by Luminița Antonela ZAGRAI, Ioan ZAGRAI, Smaranda Doina ROȘU-MAREȘ, Georgeta Maria GUZU, Claudiu MOLDOVAN, Zsolt JAKAB-ILYEFALVI, Anca Maria ȘOFRON

Ten new established sweet cherry orchards in Moldova region were surveyed in the summer of 2021 to assess the phytoviral status. Nine of these were established with abroad propagated material from Italy, Belgium, Czech Republic and Netherlands, and the other one with material produced in Romania. Two blocks of 200 trees from each orchard were visually monitored for virus-like symptoms and then ten samples per orchard were randomly collected for laboratory testing. Sampling trees were tested for the presence of the following viral pathogens: Prune dwarf virus (PDV), Prunus necrotic ring spot virus (PNRSV), Apple mosaic virus (ApMV), Apple chlorotic leaf spot virus (ACLSV), Plum pox virus (PPV), Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV) and Tomato black ring virus (TBRV) by DAS-ELISA using BIOREBA antiserum kits. The presence of Little cherry virus-1(LChV-1) on three samples which expressed symptoms of potentially infections was checked by RT-PCR. One out of ten surveyed sweet cherry young orchards found to be infected by one virus. PNRSV was detected in one orchard with an occurrence of 10%. The average of infection with PNRSV in the surveyed orchards from Moldova was 1%. No other infection was found in the surveyed orchards. The newly established orchards in Moldova region have generally a very good phytoviral status which is an important prerequisite for their success. However, there are some cases with virus infected old orchards in the proximity of the newly established orchards which might represent potential source of infection.

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