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IN VITRO IMPACT OF CONCENTRATION AND ADDITION METHODS OF PLANT HORMONS ON PEACH (Prunus persica L. Batsch) MICROGRAFTING

Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 2
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim HASAN, Ihasn AZEB, Kawther ALGHASHEEM

Florin peach variety and Myrobalan 29C rootstock were included in the experiment. For the preparation of graft and rootstock, two methods were tested for this operation: I, submerging the explants resulting from the initiation step in solutions containing NAA and IBA in concentrations (0.00 control, 0.01, 0.50, 1.00 and 2.00) mg/l respectively and II, take the explants resulting from the initiation step and grow these explants into culture media tubes containing NAA and IBA in concentrations (0.00 control, 0.01, 0.50, 1.00 and 2.00) mg/l respectively. For the application of micrografting, two methods were tested: I, Micrografting before rooting and II, Micrografting after root formation. Results show the role of IBA in rooting the explants that gave the highest rate of root number (7.00 root/explant) and root length (9.07 cm root length/explant) at all concentrations studied with the same NAA concentrations. Also, the method of submerging explants (immersion in solutions containing rooting hormones and then planting them in hormone-free culture media) was superior to the number and length of roots produced by the method of adding rooting hormones to culture media shoots.

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