Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 2
Written by Cristina IONESCU, Liliana BADULESCU, Mihaela IORDACHESCU
Prunus avium L. is a fruit trees species belonging to the Rosaceae family, cultivated worldwide in temperate climates. This study presents the genomic DNA extraction and RAPD method optimisation using the 'Severin’ sweet cherry cultivar. For genomic DNA genomic DNA extraction was optimized the method for breaking the cell wall. The parameters optimized for RAPD reaction were genomic the annealing temperature, DNA concentration and primer concentration. The best method for breaking the cell wall was Method 1, grinding the tissue with liquid nitrogen. The optimum annealing temperature was determined to be 30°C. The concentration of genomic DNA in the RAPD reaction varied between 0.05 ng/µl and 1.00 ng/µl, and that of the primer varied between 0.1 µM and 2.0 µM. The optimum concentration for the genomic DNA proved to be 0.05 ng/µl and that of the primer 2.0 µM. These results will be applied in a future experiment that will study the genetic variability of Romanian sweet cherry cultivars present in the USAMV of Bucharest orchard collection.
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