Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 1
Written by Sabin MOLNAR, Doina CLAPA, Amal DAGNI, Loredana LEOPOLD, Monica HÂRŢA, Ioan Claudiu BUNEA
The in vitro response of the blueberry cultivar Hortblue Petite to drought stress induced by PEG 6000 was investigated in this study. PEG was added to the culture media before pH adjustment at the following concentrations: 0 g/L, 10 g/L, 20 g/L, 30 g/L, 40 g/L, 50 g/L. The culture medium used was Woody Plant Medium (WPM) with 100 mg/l Sequestren 138, 1 mg/L zeatin (Z), and a pH of 5. After 80 days of in vitro culture, the average number of shoots, shoot length, fresh weight (FW), dry weight (DW), water content (WC), chlorophyll (Chl a, Chl b), carotenoids (Caro), proline (Pro), total soluble sugars (TSS), total phenolic compounds (TPC), total flavonoids (TFC), malondialdehyde (MDA), and hydrogen peroxide (H2O2) were evaluated. Drought stress induced by PEG increased the number of proliferated shoots but harmed shoot length. A reduction in the content of photosynthetic pigments was also observed. Osmolytes, oxidative stress markers, and antioxidant compounds also indicated the effect of drought stress on the Hortblue Petite blueberry cultivar.
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