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EVALUATION OF THE POLYPEHNOL EXTRACTS FROM VINE CANES (Vitis vinifera L.) OBTAINED BY AN IMPROVED EXTRACTION METHOD

Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 1
Written by Laura-Dorina DINU, George Adrian COJOCARU, Cornel BANITA, Emanuel VAMANU, Arina Oana ANTOCE

Waste vegetal materials generated during vine pruning are rich in bioactive molecules, especially polyphenols, therefore their valorization received considerable attention in the last years. This work focuses on evaluating the polyphenol composition and antimicrobial activity of vine cane extracts obtained with an improved method using a pre-treatment with cell wall degrading enzymes and fluidized bed extraction. Different quantities of canes (1 to 20 g) were used to obtain several extracts. Total phenolic content (TPC), hydrolysable and condensed tannins, antimicrobial and antioxidant activity (DPPH test) were determined. The extract obtained from 5 g of dry vine cane showed the highest antioxidant activity (85.64±0.22%), a high value of TCP (83.85±4.62 mg GAE/g dw) and a 2.44 times higher antimicrobial activity compared to antibiotic ciprofloxacin on E. coli ATCC 8739. However, the highest gallotannin content (16.26±0.03 mg tannin acid/g dw) was detected in the extract obtained from 20 g of vine canes, while the condensed tannin was low in all samples.

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