Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 1
Written by Ioana CIOBANU, Adrian George PETICILĂ, Cătălina NICOLAE, Oana VENAT
The commercial exploitation of Phalaenopsis, an epiphytic orchid, is widespread, but due to its challenging vegetative propagation, there is a necessity to enhance micropropagation techniques. This study aims to determine the optimal medium and growth regulators for the successful asymbiotic seed germination, development, and multiplication stages of Phalaenopsis. Seeds from 4-month-old-hand-pollinated orchid were sown on ½ Murashige and Skoog (MS). The resulting PLBs were transferred on 4 types of culture media:1/2 MS (1), Malmgren (MLG) media (2), ½ MS with MLG vitamins (3), ½ MS with MLG vitamins, microelements and iron at full strength (4).The fourth medium was found most suitable as it provided the highest length and overall best conditions for evolution. In the multiplication stage a variation of explants: protocorms, leaves, stems and roots were put on 3 culture media, with a variation of light condition, dark and 16/8 photoperiod. The first media did not contain growth regulators and the other two containing also 2 mg/l BAP +0.5 mg/l NAA+100 mg/lPVP+1 g/l CA, respectivly 2.5 mg/l BAP+0.75 mg/l NAA + 100 mg/l PVP + 1 g/l CA.
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