Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 1
Written by Andreea TICAN, Mihaela CIOLOCA, Monica POPA
This study has as its main objective the meristematic regeneration starting from small explants (0.1-0.2) to ensure viral eradication in potato culture. The experiment is monofactorial, the analyzed factor being the variety with 4 graduations: a1: Azaria; a2: Braşov; a3: Cosiana; a4: Cezarina (as control). For Azaria, Brasovia and Cosiana cultivars, the DAS-ELISA test was performed from potato tuber sprouts to determine the viral infection of these cultivars. Thus, the Cosiana variety presented the highest viral load, according DAS-Elisa test, 3 viruses specific to potato culture were identified: PVS, PVM and PLRV; this is followed by the Brasovia variety, which presented the PVS and PVM viruses, and for Azaria variety, the existence of PVS virus was observed. The inability to guarantee complete elimination of viral particles, especially in cases with mixed infections, remains a limitation for these methods based on meristem cultures. After each pass of the meristems, the statistical analysis was performed, regarding the regeneration of the meristems, finding after each pass the decrease in the viability of the meristems, the reason being the reduced sizes of the meristems.
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