Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 2
Written by Andreea E. MANOLESCU, Anca P. ONACHE, Carmen F. POPESCU, Dorin I. SUMEDREA, Anamaria M. DUMITRU
The Ştefăneşti-Argeş wine region is known for its unique terroir and traditional practices in viticulture and winemaking. Yeasts play a significant role in shaping wines' characteristics and fermentation processes. The surface of grape berries is a natural habitat for various microorganisms, mainly represented by yeasts, lactic acid bacteria, and acetic acid bacteria, known as the epiphytic microbiota. The aim of this work was the isolate, identify, and characterize some yeast strains from different grape varieties: five internationally well-recognized varieties for white and red wines grown in the INCDBH Stefanesti vineyard. The isolates selected in pure culture proved to belong to both Saccharomyces and non-Saccharomyces genera, an aspect confirmed by microscopic and molecular analyses. For correct identification, the morphological characteristics were complemented by molecular analyses by PCR amplification of the ITS1-5.8S-ITS2 region.
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