Published in Scientific Papers. Series B, Horticulture, Vol. LXVIII, Issue 2
Written by Catalina NICOLAE, Oana VENAT, Adrian PETICILĂ, Teodora-Elena KISS, Dorel HOZA
This study explores the effects of different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6 benzylaminopurine (BAP) on the tissue culture of Solanum tuberosum cultivar 'Blue Congo’. Nodal segments derived from in vitro shoots were cultured on 9 variants of MS medium with varying concentrations of 2,4-D (0.5, 0.7, and 1.0 mg/L) and BAP (0.1, 0.2, and 0.5 mg/L). The explants were evaluated under different light conditions to determine their viability, shoot regeneration, and micro-tuber formation. Results indicated that light conditions significantly influenced explant viability and shoot growth, with optimal shoot regeneration observed in specific hormone combinations. Additionally, micro-tuber formation was highest in certain variants. Further studies will be published to evaluate the ability of the obtained callus to produce somatic embryos and, ultimately, to develop into plantlets.
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