Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Elena Andreea VOCHIN (DUMITRESCU), Valentina ISAC, Florin STĂNICĂ
This paper present results regarding the sterilization effectiveness of plant material applied to peach and plum rootstocks explants necessary for the initiation of the in vitro culture. For peach and plum rootstocks tissue culture initiation, two pre-sterilization agents (sanitary alcohol and ethanol) and two sterilization agents (sodium hypochlorite and calcium hypochlorite) where tested in 16 different variants. The Mirobolan dwarf explants were cultured in Murashige and Skoog, 1962 basal medium and the Adaptabil explants were cultured in Querin and Lepoivre, 1977 basal macronutrients and micronutrients and Linsmaier and Skoog, 1965 vitamins. The growth chamber for the in vitro culture had 22±2˚C temperature, with a photoperiod of 16h day light and 8h dark. For Mirobolan dwarf rootstock a good survival rate of explants was obtained both in sterilization variant 15 (56.67%) and in sterilization variant 16 (63.33%).With the Adaptabil rootstock, good sterilization results were also obtained in variant 16 (70%). A good percentage of survival with a lower contamination rate was obtained also in variant 1 (69.67%). Both for Mirobolan dwarf and for Adaptabil rootstocks, the sterilizing agents from variant 16 can be used to sterilize the plant material.
[Read full article] [Citation]
