Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Ana-Maria RADOMIR, Ramona STAN, Dorin Ioan SUMEDREA
Lonicera caerulea L. (blue honeysuckle) is a valuable nutraceutical species belonging to the Caprifoliaceae family. This study aimed to develop efficient micropropagation protocols for three blue honeysuckle cultivars: 'Loni’, 'Cera’, and 'Kami’. The use of 8% calcium hypochlorite for disinfection of explants and a plain Murashige and Skoog (MS) medium for their inoculation proved effective in inducing regenerative processes. In the initiation phase, 'Loni’ had the best regenerative potential of the three cultivars studied (between 40 ± 10 and 73.33 ± 11.55% explants that developed shoots). The highest multiplication rate (4.00 ± 0.10 shoots/explant) and the highest average shoot length (7.89 ± 0.50 cm) were obtained for the 'Cera’ cultivar on MS medium supplemented with 1 mg/L BAP and 1 mg/L IAA. The most effective culture medium for in vitro shoot rooting proved to be MS½ medium supplemented with 2 mg/L IBA. The ’Loni’ cultivar showed the best rooting potential (rooting rate 93.34 ± 5.77%, number of roots/shoot 4.33 ± 0.15, and average root length 2.27 ± 0.06 cm). In vitro regenerated plants were efficiently acclimatized to ex vitro conditions. In vitro rooting can be successfully replaced with in vivo rooting simultaneously with acclimatization, thus shortening the technological flow of obtaining planting material through micropropagation and considerably reducing the unit price of the plant.
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