Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Ionela-Cătălina GUȚĂ, Elena-Cocuța BUCIUMEANU
Distributed worldwide, Pinot Gris Grapevine Virus (GPGV) is considered a major pathogen of grapevine. Virus-free
propagation material is the first step in the control of viral diseases, often requiring the application of methods to
regenerate healthy plants. In vitro chemotherapy is the sanitation method used in this work, its validation being necessary
to improve the efficiency of obtaining healthy biological material. To obtain GPGV-free grapevines, the treatment was
applied to recover valuable clones from INCDBH Ștefănești, Fetească albă 97 Șt. and Cabernet Sauvignon 131 Șt. The
apices and axillary buds were collected during the active growth period in two stages from plants grown in the protected
space and in the field. A subculture of these explants on medium with ribavirin and oseltamivir, followed by three
multiplication and one rooting on medium without antivirals, led to the regeneration of new plants with elimination rates
of 50-100%, effective elimination being achievable with a precision of 21%, independent of genotype, explant type and
sampling period.
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