Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Doina CLAPA, Monica HÂRŢA, Ana Maria RADOMIR, Adrian PETICILĂ, Dorin Ioan SUMEDREA
Meta-Topolin (mT), a compound from the cytokinin class that promotes plant growth and development, is characterized by lower toxicity than other cytokinins. This study explores the effect of mT on the in vitro multiplication of Hypericum perforatum L., Mentha x piperita L., and Stevia rebaudiana Bertoni. After six subcultures on Driver and Kuniyuki Walnut (DKW) medium supplemented with 2 mg/L mT, growth parameters were evaluated, including the number of shoots per explant, shoot length, the number of roots per explant, and root length. In the presence of mT, S. rebaudiana produced the highest number of shoots per explant (4.2 ± 0.12), while the longest shoots were recorded in M. piperita (6.2 ± 0.68 cm). The highest in vitro rooting percentage was observed in M. piperita, reaching 80%. The rooted shoots were acclimatized in perlite. Molecular analysis using Start Codon Targeted (SCoT) and Inter simple sequence repeat (ISSR) markers confirmed the genetic fidelity of the acclimatized plants compared to the mother plants, affirming the stability of in vitro cultures using mT as a cytokinin source.
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