Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Mihaela Irina HOLOBIUC, Alexandra-Gabriela CIOCAN, Rodica CATANÄ
Plant biotechnology ensures the production of a wide range of compounds for industry, pharmacy, cosmetics and medicine. Tissue cultures can provide useful metabolites all-round the year under controlled conditions with reduced expenses. L. nivale subsp. alpinum (Cass.) is a protected taxon, of ornamental value and economic importance. Our aim was to produce highly regenerative cultures for mass production and synthetic seeds development, as mean for multiplication and preservation. The effect of different growth factors was studied concerning regeneration through direct morphogenesis and somatic embryogenesis. The presence of 2.4-D and Dicamba alone or combined with low levels of kinetin favoured somatic embryogenesis. Histological samples of embryogenic aggregates were analysed using optical and scanning electron microscopy. Somatic embryogenesis provides a large mass of plant material for basic studies and for economic purposes. For synthetic seeds production, embryogenic aggregates of 2-3 mm diameter were incubated in 3% sodium alginate in a modified CaCl2-free MS liquid medium. Subsequently, dehydrated seeds were cultured on MS agar solidified medium added with growth regulators to test the artificial seeds’ growth.
We improved the regeneration rate through somatic embryogenesis and, subsequently, produced artificial seeds useful for multiplication and conservative purposes.
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