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STUDY ABOUT THE INFLUENCE OF GIBBERELLIC ACID AND NAPHTHYL ACETIC ACID ON IN VITRO DEVELOPMENT OF FOUR ROMANIAN POTATO VARIETIES

Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 1
Written by Andreea TICAN, Mihaela CIOLOCA, Monica POPA

In 2024 within the Research Laboratory for Plant Tissue Cultures of National Institute for Research and Development for Potato and Sugar Beet Brașov, Romania an in vitro study was performed to determine the optimum concentration of gibberellic acid and naphthyl acetic acid for growth and development of plantlets belonging of four Romanian potato varieties, created at our institute. The minicuttings were cultured in Murashige and Skoog medium (MS). Gibberellic acid and naphthyl acetic acid were applied in different concentrations. The bifactorial experience of the 4*4 type included 16 variants, on 3 repetitions. Factor a is represented by the variety with 4 graduations (Azaria, Brașovia, Cosiana and Cezarina) and factor b is the culture medium with 4 graduations (0 mg GA3: 0.005 NAA; 3 mg GA3: 0.005 mg NAA; 4 mg GA3: 0.01 mg NAA; 5 mg GA3: 0.2 mg NAA). Measurements were made for: number of leaves, vitroplants height, root length and internodes number. For the analyzed parameters, the varieties behaved differently with each hormonal treatment applied.

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