Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 2
Written by Rodica CATANĂ, Raluca MIHAI, Larisa FLORESCU, Monica MITOI
Negru Vârtos is a variety of Vitis vinifera cultivated since the Dacian period. It is one of the most appreciated varieties in Romania, giving it a balanced and intensely colored wine. Our research aimed to increase the production of secondary metabolites in a long-term V. vinifera cv. Negru Vârtos callus culture obtained many years ago. Seven different abiotic factors represented by different media composition, salt addition, and UV or low-temperature exposure were tested to achieve this goal. The phenolic, flavonoid, anthocyanidin, and proanthocyanidin content was determined. Also, antioxidant activity was estimated by the α-diphenyl-α-picrylhydrazyl free radical-scavenging method (DPPH). Although all the tested variants showed better results concerning the callus morphometrical parameters as callus survival percentage, callus growth index, or callus moisture, only the variants treated with a low temperature of 4ºC for 24 h revealed an increase of flavonoids, proanthocyanidins, and anthocyanidins content. Also, the production of anthocyanin was slightly stimulated in the R2 variant.
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