Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 2
Written by Ioana KLEE, Lenuţa CHIRA, Daniel NAGY, Constantin PĂUN, Ligia ION
Because, for medicinal and aromatic plants there is the ease of being genetically modified both naturally through mutations and through external intervention. In this context, the effectiveness of the present protocol to produce good quality DNA suitable for the detection of genetically modified crops was evaluated. Genomic DNA isolated from different parts of coriander, fennel and sage plants through was done. For this, through the PCR technique, three molecular markers were used to amplify the neomycin phosphotransferase gene (nptII), which is used as a marker gene in mutation identification processes. The existence of nptII (target of 173 bp) was investigated in the plant material studied in the 3 plant species (coriander, fennel and sage), vegetative parts (leaves) but also the seeds considered in this work. Successful PCR amplification of the nptII gene with a randomly amplified polymorphic DNA primer and complete digestion of the isolated DNA with the restriction enzyme HindIII validated the quality of the isolated DNA.
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