Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 2
Written by Ahmed MEHMED, Stanimir ENCHEV, Tzvetan KIKINDONOV
In the conditions of Bulgaria, Stevia is propagated by planting seedlings. Seedlings are produced by cuttings from stored rhizomes, from seeds and from in vitro micropropagation and rooting techniques. In the laboratory of tissue cultures of the Agricultural Institute - Shumen, methods have been developed for efficient preservation of elite clones and micropropagation. In the 2020-2023 study, the influence of genotype on the proportion of rooting in vitro was evaluated. A comparative analysis of the survival of rooted in vitro regenerants during their adaptation to external conditions was performed, compared to that of cuttings from rhizomes and propagation from seeds. Seedlings are qualified according to biometric parameters - length and mass of the stem and roots. In vitro techniques remain the safest method for preserving and propagating elite material of Stevia for Bulgaria's conditions.
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