Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 2
Written by Ana-Maria RADOMIR, Ramona STAN, Doina CLAPA, Adrian PETICILĂ, Andreea Elena MANOLESCU, Dorin Ioan SUMEDREA
Thymus x citriodorus (Pers.) Schreb. (lemon thyme) is a perennial aromatic plant valued for its culinary, medicinal, and ornamental properties. This study aimed to establish an efficient micropropagation protocol for lemon thyme and to evaluate the genetic fidelity of in vitro regenerated plants using RAPD molecular markers. The highest shoot multiplication rate (13.77±2.51 shoots/explant) was obtained on Murashige and Skoog (MS) medium containing 3 mg/L BAP. The longest shoots (3.76±0.46 cm) were generated in the presence of 0.5 mg/L 2iP and 5 mg/L GA3. Rooting was most efficient (100% rooting rate) on ½MS medium supplemented with IBA (0.5 or 1 mg/L) and in the presence of IBA (0.5 or 1 mg/L) in combination with GA3 (3 mg/L). Acclimatized plants showed a 100% survival rate. Molecular analyses revealed monomorphic banding patterns in RAPD profiles, confirming the genetic fidelity of micropropagated plants.
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