Published in Scientific Papers. Series B, Horticulture, Vol. LXV, Issue 1
Written by Mirela Irina CORDEA, Rodica POP
The Ipomoea platensis species belongs to the Convolvulaceae family. It grows as a wine and is widespread in tropical and subtropical areas. This morning glory is a caudiciform species, with tuberous roots, which also grow on the surface of the soil, thus having a good resistance to drought. In Romania’s climatic conditions this species is flowering if fertilized but the capsule (bud) aborts usually before seed maturation, therefore the vegetative propagation is the only way to multiply it. In this research we investigated its optimum vegetative multiplication (ex vivo and in vitro). For ex vivo multiplication the best results were obtained using tap water without growth hormones. For in vitro multiplication we used nodal segments and leaf fragments. The nodal segments used as explant produced shoots, while the leaf fragments developed only callus. The explants were placed on MS medium containing cytokinines (BAP, KIN and TDZ) and auxins (IAA and IBA) in different concentrations. Optimal proliferation was observed when shoots were cultivated on MS medium supplemented with 0.5 mg/l IBA and 0.2 mg/l KIN. The concentration and type of cytokinine had an influence on the multiplication of I. platensis species, from nodal segments. Thus, Kinetin in 0.2 mg/l concentration induces the formation of a higher number of shoots compared to the cytokinines BAP in 0.3 mg/l and TDZ in 0.1 mg/l concentration. Consequently, the easiest way to multiply this species is ex vivo, but the highest ratio of multiplication is obtained in vitro, from nodal segments.
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