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MORPHOMETRIC AND MOLECULAR CHARACTERIZATION OF PARATYLENCHUS NANUS COBB, 1923 (TYLENCHIDA: PARATYLENCHIDAE) ASSOCIATED WITH SOIL FROM GLADIOLUS PLANTS

Published in Scientific Papers. Series B, Horticulture, Vol. LXV, Issue 1
Written by Mariana RĂDOI (DUMITRU), Anca Cristina BIGHIU, Florin TOMA

Nematodes belonging to the genus Paratylenchus are widespread throughout the world and are associated with many plant species. Morphological identification of Paratylenchus species is a difficult task because it relies on many characters with a wide range of intraspecific variation. In this study we provided morphological and molecular characterisation of the species Paratylenchus nanus. In 2019, 5 soil samples were taken from Argeș county from different flowering plants to detect and identify phytoparasitic nematodes. Soil samples were taken from the following species of flowering plants: Lilium, Chrysanthemum, Gladiolus and Lisianthus. Based on laboratory tests, the nematode Paratylenchus nanus was detected in a soil of Gladiolus plants. Species were identified by morphobiometric analysis and molecular biology. To confirm the species, sequencing and phylogeny analyses were performed. Thus, following this study, we report for the first time, in Romania, the presence of the nematode Paratylenchus nanus, on another host plant, than the one previously reported.

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