Published in Scientific Papers. Series B, Horticulture, Vol. LXV, Issue 1
Written by Ioana-Cătălina NICOLAE, Dorel HOZA, Adrian George PETICILĂ, Oana VENAT
Synthetic seed technique can be a useful tool in plant conservation, as it combines the advantages of vegetative and generative propagation: uniformity of the material, lower costs for storage and transportation and the production of pathogen-free planting material. Kalanchoe blossfeldiana in vitro derived explants were used to examine the influence of sucrose concentration in the encapsulation maxtrix and the influence of salt concentration in the storage medium on the conservation of this species. Nodal segments and shoot tips were encapsulated in alginate solution containing two different sucrose concentrations (2% and 4%) and stored for 7,30 and 60 days in liquid medium with different MS salt concentrations (normal, halved and quartered). Statistical tests did not reveal an influence of the sucrose concentration in the alginate solution. On the other hand, tests indicated that the variants with the low salt concentrations (halved and quartered) showed better growth in terms of average shoot length and average number of leaves.
[Read full article] [Citation]
