Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 1
Written by Doina CLAPA, Monica HÂRŢA
The aim of this research was to investigate the influence of cultivar upon in vitro multiplication rate of highbush blueberry (Vaccinium corymbosum L.) and to evaluate the genetic fidelity of in vitro-propagated plants using molecular markers. Four varieties of blueberries were studied: 'Bluecrop’, 'Blueray’, 'Brigitta’, and 'Duke’. For the in vitro multiplication, the Woody Plant Medium (WPM) basal medium was used, supplemented with 1 mg/L zeatine, 100 mg/L Sequestrene 138 and Plant agar 4 g/L, pH = 5. After ten weeks of in vitro culture, the four highbush blueberry varieties had average proliferation rates between 2.98 ± 0.25 and 9.35 ± 0.50 and the average length of the shoots varied between 2.79 ± 0.15 cm and 3.29 ± 0.13 cm. Clonal fidelity has been checked by twelve Start Codon Target Polymorphism (SCoT) primers. No polymorphism was detected, that proving that the regenerated plants showed high clonal fidelity.
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