Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 1
Written by Georgeta Maria GUZU, Ioan ZAGRAI, Luminița Antonela ZAGRAI, Claudiu MOLDOVAN, Mirela Irina CORDEA
Apple is one of the most important crops in temperate areas, and it is situated on the second place, after plum, in Romania. The scab caused by Venturia inaequalis is a widespread disease in apple crop, which produces considerable economic losses. The aim of the study was to identify the presence of the Vf gene in four local apple cultivars ('Goldprim', 'Dany', 'Doina' and 'Alex'), obtained by the FRDS Bistrita. The investigated cultivars are known to be resistant to scab, but their selection was made by classical methods which do not guarantee the presence of Vf gene. To get an accurate confirmation of the Vf resistance mechanism in the genome of the cultivars, a molecular tool was used. Thus, the mentioned apple cultivars were tested by using the Marker Assisted Selection (MAS) method, with three pairs of specific markers: AL07, which allows the producing two bands corresponding to Vf-dominant, and vf-recessive respectively; the other two markers are AM19 and U1400 as dominants (Vf). According to the results obtained, the Vf resistance genes were identified as heterozygous (Vfvf) in all four local apple cultivars tested.
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