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GENETIC RELATIONSHIPS BETWEEN SEVERAL ROMANIAN PLUM VARIETIES USING RAPD MOLECULAR MARKERS

Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 2
Written by MIHAELA IORDACHESCU, Liliana BĂDULESCU, Adrian ASĂNICĂ, Anca Amalia UDRIȘTE

Plum (Prunus domestica L.) is the dominant fruit tree species in Romania and has an ancient tradition in growing all over the country. The assortment of the plum varieties has known a continuous increase in time, enhancing its genetic variability. In the current work, RAPD analysis was carried out in order to assess the genetic relationships between seven Romanian plum cultivars ('Brumării de Voinești’, Record’, 'Gemenea’, 'Elena’, 'Centenar’, 'Silvia’, and ’Pescăruș’) existent in the germplasm collection of the Faculty of Horticulture, USAMV Bucharest, Romania. Five random decamer primers identified 28 polymorphic and 11 monomorphic RAPD loci. The constructed UPGMA dendrogram associated the cultivars studied into 2 clusters, one cluster with the varieties 'Record’ and 'Gemenea’, and another cluster grouping the rest of the varieties. The genetic fingerprints obtained following amplification with the RAPD markers, are specific for each variety, and may be used for molecular identification of the varieties in the germplasm collection.

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