Published in Scientific Papers. Series B, Horticulture, Vol. LXVI, Issue 2
Written by Oana VENAT, Adrian-George PETICILĂ, Cătălina-Ioana NICOLAE, Cristian-Mihai POMOHACI, Dorel HOZA
Solanum tuberosum and Ipomoea batatas are the third and the seventh world ranking species in terms of yield and consumption and they are relatively cultivated based on micropropagation techniques for limiting viruses spreading and other diseases. In this work we analyzed varieties of Ipomoea batatas, 'Ro-Ch-M’, 'KSH’ and 'KSP1’, two varieties of Solanum tuberosum L. with purple flesh, 'Violet Queen’ and 'Purple Majesty’ and their response to variation of auxins and cytokinins on in vitro cultivation. The study compared the effects of basal MS medium containing various concentrations of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in combination with gibberellic acid (GA3) in micropropagation of those species, using material from a starter culture in vitro induced as well. For Solanum tuberosum, the shoot induction ranged between 4–5 days with variation among NAA concentrations, the longest shoots (9,8 cm), maximum number of nodes (4-5), and maximum number of leaves (10.00) were recorded on 'Purple Majesty’ on variant containing 0,25 mg/L BAP + 0.03 mg/L NAA+0,05 mg/L GA3. For Ipomoea batatas, the shoot induction ranged between 5-6 days with with variation among NAA concentrations, the longest shoots (7,8 cm), maximum number of nodes (3.8 cm), and maximum number of leaves (12.00) were recorded on 'KSP-1’ on variant containing 0,25 mg/L BAP + 0.05 mg/L NAA+0,05 mg/L GA3. The results showed that the combined effect of various concentrations of NAA between 0.01 mg/l and 0,05 mg/L, BAP between 0,25 mg/L and 1.00 mg/L and GA3 could provide solution for extend in vitro production of Solanum tuberosum tubers and potatos for Ipomoea batatas as base materials for industrial cultivation.
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