Published in Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 1
Written by Doina CLAPA, Monica Hârța, Claudiu Ioan BUNEA, Lehel LUKÁCS, Eugenia HĂRȘAN, Gabriela ROMAN, Peter Alpar SOMSAI
This study was carried out to investigate the effect of three cytokines [6-benzyladenine (BA), meta-Topoline (mT), and zeatin (Z)] on the in vitro propagation of C. avellana. The culture medium used in all in vitro culture stages was McCown's Woody Plant Medium (WPM) supplemented with 100 mg/l Sequestrene 138.3% (w/v) sugar, solidified with 5 g/l. Plant agar and pH 5.8. At the initiation stage, single-node mini cuttings were inoculated on WPM medium supplemented with 2 mg/l Z, and the initiation percentage was 66.66%. The highest proliferation rate (2.21 ± 0.11) was observed on the culture medium supplemented with 4 mg/l BA followed by the culture medium supplemented with 4 mg/l Z (1.93 ± 0.03). The shortest shoots were measured on WPM medium with 4 mg/l BA, with an average length of 1.81 ± 0.08 cm, and the longest shoots (3.46 ± 0.02 cm) were obtained on WPM medium with 4 mg/l Z. The in vitro rooting percentage was 81.66 ± 0.39% when using the WPM medium with 1 mg/l Z. The rooted plants obtained in vitro showed an acclimation rate of 97.66%, and out of these, 96% survived under greenhouse conditions.
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