Published in Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 1
Written by Rodica POP, Doru PAMFIL, Monica HÂRŢA, Doina CLAPA, Mirela Irina CORDEA
Micropropagation of five grapevine cultivars (Fetească albă, Cabernet Sauvignon, Merlot, Riesling Italian and Traminer roz) was performed using axillary shoots as a source of explant from field-grown plants. Shoot initiation and proliferation were performed on Murashige and Skoog (MS) medium with 0.5 mg/L NAA and BAP (0.5, 1.0 and 2.5 BAP mg/L). Subsequently, proliferated shoots from V3 were used for grapevine callus induction. The callus was then subcultured on fresh MS medium supplemented with 1.0 mg/L BAP +5.0 (V4) and 10 mg/l NAA (V5). Embryogenic calli grown on V5 were transferred and subcultured on MS medium supplemented with 0.5 mg/L NAA and three different concentrations of TDZ (0.5; 1.0; 2.0 mg/L) for shoot regeneration. RAPD assay was performed after the 12th subculture using in vitro-raised plants from V6 culture medium (0.5 mg/L NAA+0.5 mg/L TDZ) and the mother plants grown in the field and used as control. The results of this study reveal that the highest number of somaclones (5) was regenerated from the Merlot variety and the lowest number (1) from the Feteasca albă and Traminer roz varieties. For practice, the induction of somaclonal variation and the selection of valuable somaclones are important goals for future grapevine breeding programs.
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