Published in Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 1
Written by Marina SAMIEE, Monica HÂRŢA, Doina CLAPA, Mihai BOTU
In this study, eleven willow genotypes from local populations and foreign varieties grown in an experimental field were morphologically and genetically evaluated to establish their genetic diversity. The results of this study show that the local clonal selections Caracal 101-103 recorded the best results for most of the analysed morphological characters. Out of 25 SCoT primers tested for genetic diversity analysis, 12 primers generated 113 polymorphic bands. The lowest number (8) was obtained with primers SCoT1, SCoT3, SCoT21 and SCoT23. The highest number (13) of polymorphic bands was amplified with the primer SCoT13. The percentage of polymorphism ranged from 72.72% (SCoT 1 and SCoT 21) to 100.0% (SCoT 13) with a mean value of 83.69%. The UPGMA dendrogram grouped willow genotypes into two main clusters. The results of the cluster analysis were then confirmed by the PCoA analysis which explained 61.76 % of the total variation. In conclusion, this study provides valuable data regarding the genetic diversity of willow genotypes that can be selected and used in future breeding programs.
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