Published in Scientific Papers. Series B, Horticulture, Vol. LXVII, Issue 2
Written by Mihaela IORDĂCHESCU, Cristina IONESCU, Liliana BĂDULESCU
Sweet cherry, Prunus avium L. belongs to the Rosaceae family, and nowadays is cultivated worldwide in temperate climates. Currently, plant breeders are using molecular markers, such as RAPD to reduce the time required to create new hybrids and valuable varieties. In the present study, seven RAPD markers were used to study the genetic variability and genetic relationships between 5 sweet cherry genotypes present in the orchard collection of USAMV Bucharest, 4 Romanian ('George’, 'Ludovan’, 'Bucium’, 'Maria’, Iașirom’, Cociuvaș’, 'Alexus’, 'Andreiaș’, 'Rubin’, 'Severin’, 'Paulică’, 'Boambe de Cotnari’, 'Cetățuia’, and 'Putna’) and the Swedish cultivar 'Rivan’. All RAPD markers proved to be polymorphic, allowing for amplification of 44 loci, with P63 amplifying the highest number of loci (27). The UPGMA dendrogram build on RAPD data grouped the genotypes studied into 2 clusters, one cluster containing mostly descendent of 'Van’ and 'Boambe de Cotnari’, and the second cluster grouping varieties with German genitors. The present study demonstrates the genetic variability among the Romanian genotypes present in the USAMV of Bucharest orchard collection.
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