Published in Scientific Papers. Series B, Horticulture, Volume LXII
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT
In vitro propagation is one of the most convenient methods for plant material multiplication in order to obtain virus free planting material in high quantity and short time. The paper presents the influences of genotype, growth season and hormones on in vitro propagation of some new Romanian peach varieties with very good prospects on the market. Three peach genotypes: ʻFlorinʼ, ʻFilipʼ and ʻMimiʼ from the Didactic Field of Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine of Bucharest were tested. In the protocol for the in vitro establishment, winter and spring growth season were chosen. Initial shoot explants were obtained in winter by forcing the dormant shoots in the growing chamber and in spring (April-May), directly from the field. The explants were cultured on MS (Murashige and Skoog, 1962) basal medium supplemented with 30 g sucrose and 7 g agar, as carbon source. Benzyl aminopurine (BAP) on four variants with 0, 1, 5 and, respectively, 10 mg/l was used as cytokinine. The results show that Florin genotype was superior to the rest of the varieties used in the experiment in terms of the number of formed shoots and the strength of their growth. For the in vitro culture initiation, the shoots taken in spring, during the growth season, gave the best results compared to the winter period. The results showed a significant correlation between the concentration of BAP and the shoots number (multiplication rate) and height. The concentration of 5 mg/l BAP (V3), gave the best rate of shoot formation and the highest elongation rate.
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