Published in Scientific Papers. Series B, Horticulture, Volume LXII
Written by Filiz HALLAÇ TÜRK, E. Sema ÇETİN, Zehra BABALIK, Nilgün GÖKTÜRK BAYDAR
Purpose of this study was to determine total phenolic content, phenolic composition, antioxidant and antiradical activities of ʻSenirkent Karasıʼ grape cultivar’s skin and seeds. While total phenolic contents of grape skin and seeds were determined by Folin-Ciocalteu method spectrophotometrically expressing the results in terms of gallic acid (GAE), phenolic composition was analyzed by HPLC (High Performance Liquid Chromatograph). Antioxidant activities of the grape skin and seeds were evaluated by reducing powers whereas antiradical activities were examined using DPPH (1-diphenyl-2- picrylhydrazyl). Results showed that total phenolic contents of seeds and skin were 52.32 and 1.89 mg g-1 GAE g-1 DM, respectively. Antiradical activities of seed and skin extracts (100 ppm) were 95.90 and 16.22 %, respectively. Reducing powers of seeds were 1.64 at 250 ppm, and were 2.39 at 1000 ppm whereas antioxidant activities) of skins were 0.08 at 250 ppm; 0.31 at 1000 ppm. Results showed that skin had higher amount of phenolic compounds than seeds and gallic acid, catechin, cafeic acid, syringic acid, resveratrol, quarcetin, kaempherol, p-cumaric acid were present in skin whereas only gallic acid, catechin, epicatechin were present in seeds. Seeds had the highest values of epicatechin (746.94 µg g-1) while skins had the highest values of syringic acid (17.01µg g-1 ) and gallic acid (5.29 µg g-1).
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