Published in Scientific Papers. Series B, Horticulture, Vol. LXIII, Issue 1
Written by Ileana MICLEA, Alexandra URDA, Marius ZĂHAN
This research focused on in vitro propagation of Drosera kaieteurensis and Drosera intermedia using several growth regulators. Half-strength Murashighe-Skoog medium (1/2MS) was employed for all treatments and controls. D. kaieteurensis plantlets were placed in medium supplemented with 0.5 mg/l kinetin (KIN) or 0.05 mg/l KIN+0.05 mg/l αnaphthaleneacetic acid (NAA). Leaves were transferred to 1/2MS containing 2 mg/l activated charcoal (AC) and either 10 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) or 10 mg/l 6-benzyladenine (BA) and grown in darkness (D) or under an 18/6h light/dark regime (L). D. intermedia plantlets and leaves were cultured in zeatin (Z) (0.1, 0.5 mg/l) or KIN (0.05, 1 mg/l) alone or in combination with NAA (0.05, 0.1, 1 mg/l). After 8 weeks, D. kaieteurensis plantlets developed best in the absence of growth regulators. When leaves were employed 10 mg/l BA+AC+L ensured maximum plant size while shoot formation was optimal in 10 mg/l 2,4-D+AC+D and root growth in the control medium. For D. intermedia plantlets, the lack of growth regulators resulted in larger plants and 0.1 mg/l Z stimulated significant root and shoot multiplication. Shoot and root production in both species was better when using plantlets.
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