Published in Scientific Papers. Series B, Horticulture, Vol. LXIV, Issue 2
Written by Nataliya DIMITROVA, Viktorija NIKOLOVA, Diyana ALEKSANDROVA, Lilyana NACHEVA
Acclimatization is one of the key steps in the success of micropropagation of woody plants. Improvement of acclimatization of micropropagated woody fruit plants could be achieved in different ways. The purpose of this study was to optimize the acclimatization of micropropagated sweet cherry rootstock GiSelA 6 (Prunus cerasus 'Schattenmorelle’ × Prunus canescens) through application of biostimulators of natural origin in floating system. A new generation of plant growth biostimulators Regoplant and Charkor (Agrobiotech, Ukraine) contain metabolism products of symbiotic fungus-endophyte of ginseng roots, cultivated in vitro. Micropropagated and rooted plantlets from cherry rootstock GiSelA 6 (Prunus cerasus 'Schattenmorelle’ × Prunus canescens) were acclimatized in a floating system with 100 μl L-1 Regoplant or Charkor. As control plantlets with no additional treatments served. Multi-cell bedding plant trays filled with peat: perlite 1:1 (v:v) were used for acclimatization. Plants were grown in a greenhouse under high humidity conditions for one week and then humidity was gradually decreased. Data on some growth, physiological and biochemical parameters were collected 45 days after transplanting to ex vitro conditions. Regoplant (100 μl L-1 ) led the highest plants survival rate (86%), also the greatest values for the growth determined parameters.
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