Published in Scientific Papers. Series B, Horticulture, Vol. LXVIV, Issue 2
Written by Monica HÂRŢA, Rodica POP, Liliana ROTARU, Lucia Cintia COLIBABA, Alin DOBREI, Steliana Paula BARBU, Doina CLAPA
Romania has a valuable wine heritage, and the genetic evaluation of grapevine genotypes from Vitis germplasm collections is a national priority. Thus, this study aimed to characterize 22 Romanian grapevine genotypes and five international reference varieties using nine SSR (Simple Sequence Repeats) loci. VVMD32 was the most informative (He=0.837; PI= 0.040) SSR marker with 11 alleles generated. Conversely, the VvZag62 locus generated the lowest number of alleles (6) with values recorded for He=0.744 and PI= 0.109. The genetic SSR profiles were used to construct a barcode design to compare the number and size of alleles among the screened grapevine samples. The results of this study show that SSR genotyping supplemented with a useful data grouping mode is effective for genetic diversity analysis of Vitis germplasm resources.
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